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- Original Article Effect of Cadmium and Zinc on IL-6 Secretion in a Human Monocytic Cell Line, THP-1
- Dong Hoon Shin, Suk Kwon Suh, Seong IL Suh
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DOI: https://doi.org/10.35371/kjoem.1999.11.3.332
Published online: September 30, 1999
1Department of Preventive Medicine and Public Health, School of Medicine, Keimyung University, Korea. 
2Department of Microbiology, School of Medicine, Keimyung University, Korea.
2Department of Microbiology, School of Medicine, Keimyung University, Korea.
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Abstract
OBJECTIVES
This study was performed to investigate effects of cadmium and zinc on IL-6 secretion using human monocyte in a culture system.
METHODS
We have used frypan blue dye exclusion methods to examine the effect of cytotoxicity of CdCl2, ZnCl2 in THP-1 cells. IL-6 was measured by the ELIBA method in the cell culture supernatants and the expression of IL-6 mRNA was examined by reverse transcription-polymerase chain reaction.
RESULTS
IL-6 production by THP-1 cells were decreased at 0.1 mM cadmium concentration(p < 0.05) and increased as zinc concentration increased. Zinc prevented cadmiuminduced suppression of IL-6. and the addition of a chelating agent, EDTA, restores IL-6 secretion. The expression of IL-6 mRNA decreased at 0.08 mM and 0.1 mM cadmium concentrations.
CONCLUSION
This results suggests that cadmium depressed production and gene expression of IL-6 in stimulates human monocytes, and zinc prevented cadmium-induced suppression of IL-16.
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