Annals of Occupational and Environmental Medicine

Original Article

The Effects of Diethyldithiocarbamate on the Metabolism and Hepatotoxicity of Trichloroethylene: Byung Sun Choi, Jung Duck Park, Yeon Pyo Hong; Korean Journal of Occupational and Environmental Medicine 2002;14(3):257-269. Published online September 30, 2002; DOI: https://doi.org/10.35371/kjoem.2002.14.3.257

OBJECTIVES
The purpose of this study was to evaluate the trichloroethylene (TCE) metabolism, acute toxicity, and the effects of diethyldithiocarbamate (DDTC) on the acute toxicity in TCE-intoxicated rats.
METHODS
TCE was administered orally at doses of 600, 1,200 and 2,400 mg/kg of body weight following pretreatment with either saline or 500 mg/kg of DDTC. 12 hours after administration of TCE, the concentrations of TCE, trichloroacetic acid (TCA) and trichloroethanol (TCEOH) in the blood and solid organs, and the histopathological changes in each organ were examined.
RESULTS
The level of CYP2E1 markedly decreased in the DDTC-pretreated groups. The CYP2E1 content in the TCE-treated rats increased in a dose-dependent manner. The concentrations of TCE and TCEOH were highest in the liver, and the level of TCA was highest in the blood. The DDTC-pretreated rats had a markedly increased level of TCE and decreased levels of TCA and TCEOH, than the rats pretreated with saline. These findings indicated that CYP2E1 was important in the metabolism of TCE. From the histopathological findings, centrilobular necrosis was observed in the livers of the TCE-treated rats, but no significant change was found in those rats pretreated with DDTC.
CONCLUSIONS
DDTC is considered to be effective in protecting TCE-induced hepatic damage because it inhibits the TCE metabolism.

Citations

Citations to this article as recorded by

Assessment of Volatile Organic Compounds in Blood and Urine among Residents around Camp Carroll
Hyun-Sul Lim, Wonho Yang, Geun-Bae Kim, Young-Sung Cho, Young-Sun Min, Kwan Lee, Duk Hee Lee, Young-Su Ju, Sunshin Kim, Jung Heo, Dayoung Jung
Journal of Korean Society of Occupational and Environmental Hygiene.2016; 26(1): 11. CrossRef
Case report of renal cell carcinoma in automobile manufacturing factory worker due to trichloroethylene exposure in Korea
June-Hee Lee, Inah Kim, Hongdeok Seok, Inhyo Park, Jungho Hwang, Jae-Oh Park, Jong-Uk Won, Jaehoon Roh
Annals of Occupational and Environmental Medicine.2015;[Epub] CrossRef

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Original Article

Effects of Genetic Factor, Life Styles, and Diet on Urinary Hippuric Acid Excretion in Toluene Exposed Workers: Hyeon Woo Yim, Chung Yill Park, Young Man Roh, Kang Sook Lee, Jung Min Lee, Won Chul Lee, Se Hoon Lee, Chee Kyung Chung; Korean Journal of Occupational and Environmental Medicine 2000;12(3):405-420. Published online September 30, 2000; DOI: https://doi.org/10.35371/kjoem.2000.12.3.405

Abstract PDF

OBJECTIVES
The purpose was to investigate the distributions and the effects of genetic polymorphism of aldehyde dehydrogenase 2(ALDH2), cytochrome P450 1A1(CYP1A1), and cytochrome P450 2E1(CYP2E1) on the toluene metabolism.
METHODS
The subjects consisted of 160 workers who were exposed to toluene in different industries such as paint manufacturing, painting on steel and wood products, printing, bonding, and coating. The exposed toluene level was monitored by passive air sampler, and the questionnaire variables were age, sex, smoking, drinking, previous nights drinking, use of personal protective equipment, work duration, and taking benzoic acid containing food. The urinary hippurric acid collected in the end of shift was corrected by urinary creatinine concentration. The genotypes of ALDH2, CYP1A1, and CYP2E1 were investigated using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) methods with DNA extracted from venous blood.
RESULTS
The geometric mean and the geometric standard deviation of urinary hippuric acid concentration were 0. 44 g/g creatinine and 2. 80. The urinary hippuric acid concentration was significantly related to personal exposed toluene level among personal exposed toluene level, use of personal protective equipment, and benzoic acid containing food diet. The slope differences of the regression for ALDH2, CYP1A1, and CYP2El genetic polymorphism, age, smoking, and work duration tended to be significant. In multiple regression analysis, the regression coefficient of toluene, ALDH2, CYP1A1, CYP2E1 genetic polymorphism were significant.
CONCLUSIONS
Prom the above results, urinary hippuric acid level after toluene exposure was significantly affected by the genetic polymorphism of ALDH2, CYP1A1, CYP2E1. It is needed further investigation of the urinary hippuric acid level considering the effect of genetic polymorphism.

Citations

Citations to this article as recorded by

Applications of CYP-450 expression for biomonitoring in environmental health
Ho-Sun Lee, Mihi Yang
Environmental Health and Preventive Medicine.2008; 13(2): 84. CrossRef
Past and Future Applications of CYP450-Genetic Polymorphisms for Biomonitoring of Environmental Toxicants
Bitna Yi, Ji-Yeon Yang, Mihi Yang
Journal of Environmental Science and Health, Part C.2007; 25(4): 353. CrossRef

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