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Activity of Telomerase in Coal Worker's Autopsied Lung
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Jin Suk Chung, Hae Yun Nam, Byoung Yong Ahn, Kyoung Ah Kim, Young Lim
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Korean Journal of Occupational and Environmental Medicine 2002;14(4):347-352. Published online December 31, 2002
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DOI: https://doi.org/10.35371/kjoem.2002.14.4.347
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 Abstract
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- OBJECT: We measured the activity of telomerase in coal workers lung tissue and found a significant increase in telomerase activity compared to the control group. Pneumoconiosis has the characteristics of fibroblast proliferation and the accumulation of collagen,thus finally causing the pathologic changes,which lead to the irreversible and progressive fibrosis of the lungs. We hypothesized that this cellular proliferation causing irreversible fibrosis may induce some elongation of the life cycle in the chromosomes and lead to further cellular immortalization.
METHOD
8 postmortem(within 24 hours)pneumo-coniotic cases were examined and their telomerase activity was compared with that of the autopsied lungs of lung cancer patients and of accident victims without any respiratory diseases. Using the extracted ribo-nucleoprotein from pneumoconiotic nodules, telomeric repeat amplification assay (TRAP)was done.
RESULT
The pneumoconiotic lungs showed strong telomerase activity, similar to that of the lung cancer patients, while the control group showed no such activity.
CONCLUSION
Based on the results of this study, we found that coal dust-induced cellular proliferation affects telomerase-activity clinically.
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The Role of TNFalpha Gene Promoter Polymorphism in the Development of Coal Workers' Pneumoconiosis
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Byoung Yong Ahn, Kyoung Ah Kim, Hae Yun Nam, Je Hyeok Mun, Jin Sook Jeoung, Young Lim
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Korean Journal of Occupational and Environmental Medicine 2002;14(2):117-123. Published online June 30, 2002
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DOI: https://doi.org/10.35371/kjoem.2002.14.2.117
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Abstract
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- OBJECTIVES
This study was performed in order to investigate the frequency of the TNF2 allele in patients with coal workers pneumoconiosis (CWP).
METHODS
We compared the genotype distribution of TNFalpha gene promoter polymorphism between 80 CWP patients and 54 healthy controls.
RESULTS
The results were as follows : 1. The rare allele TNF2 was significantly more frequent in CWP patients (20.6 %) than in controls (10.2 %). 2. The spontaneous or LPS-induced release of TNFalpha from the peripheral monocytes was slightly increased in the TNF2 group, but these values were not significantly different between groups. 3. In the CWP TNF2 group, the increase of LPS-induced TNFalpha release was significant in comparison with that of the controls.
CONCLUSIONS
From the above results, we suggest that the TNF2 allele is strongly associated with susceptibility to CWP development.
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Autoregulation of Quartz-induced iNOS by iNOS-derived Hydrogen Peroxide in Rat2 Fibroblast
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Kyoung Ah Kim, Hae Yun Nam, Je Hyeok Mun, Jin Sook Jeong, Young Lim, Chee Kyung Chung
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Korean Journal of Occupational and Environmental Medicine 2001;13(4):385-392. Published online December 31, 2001
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DOI: https://doi.org/10.35371/kjoem.2001.13.4.385
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Abstract
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- OBJECTIVES
This study was performed in order to investigate the molecular mechanism regulating nitric oxide synthase(NOS) induced by alpha-quartz in Rat2 fibroblast.
METHODS
alpha-quartz-induced nitric oxide(NO) and H2O2 formation and alpha- quartz-induced iNOS protein expression in Rat2 fibroblast were monitored. With iNOS inhibitor(L-N6- (1-iminoethyl)lysine hydrochloride, L-NIL) or antioxidant(catalase), we observed NO and H2O2 formation and iNOS protein expression in Rat2 fibroblast stimulated with alpha-quartz.
RESULTS
alpha-quartz stimulated iNOS-induced NO and H2O2 formation in Rat2 fibroblast. L-NIL inhibited H2O2 formation and iNOS protein expression by alpha-quartz in Rat2 fibroblast. Pretreatment with catalase blocked the autoinhibitory pathway of iNOS by iNOSinduced H2O2, therefore H2O2 and NO production and iNOS protein expression were increased in Rat2 fibrobalst stimulated with alpha-quartz CONCLUSIONS: alpha-quartz-induced iNOS stimulated H2O2 formation in Rat2 fibroblast. INOS-induced H2O2 by alpha-quartz plays an important role in the autoinhibition pathway for regulating the iNOS function in Rat2 fibroblast
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