Annals of Occupational and Environmental Medicine

Im Goung Yun

16 Articles

Cytotoxicity and Apoptosis by Silica, Asbestos and Man-Made Mineral Fibers: Young Lim, Kyoung Ah Kim, Heung Nam Kim, Dong Won Lee, Won Seop Cho, Im Goung Yun; Korean Journal of Occupational and Environmental Medicine 1997;9(4):641-649. Published online December 31, 1997; DOI: https://doi.org/10.35371/kjoem.1997.9.4.641

Exposure to various particles and fibers can result in lung inflammation that may progress to fibrosis, even lung cancer for which there is no effective clinical treatment now. The mechanism involved in pulmonary injury has not been well defined ; however, most current evidence implicates a central role for alveolar macrophages (AM) in this process. Also apoptosis or programmed cell death is regarded as a mechanism which is related with the pulmonary fibrosis. We propose that the cytotoxic potential of various particles may be evaluated by measuring lactic dehydrogenase (LDH) from particle co-cultured supernatant and theses particles may induce the characteristics of apoptosis, DNA ladder. We analyzed rat AM culture media which was incubated for 3 days with the same concentration (10 ug/ml) of silica(Si), chrysotile(Ch), crocidolite(Cr), ceramic fiber(CF), rock wool(RW) and glass wool (GW). And each particles (50ug/cm(2)) was incubated with A549 (pneumocyte in tracheal epithelium) cell lines for 24 hours to confirm the DNA ladder. Additionally, silica induced apoptosis in vivo was confirmed by electromicroscopic observation. The results were as follows; 1. Silica, asbestos and man-made mineral fibers (MMMF) co-cultured with AM showed the increase of LDH significantly with the time interval of 24, 48, 72 hours except for ceramic fiber in 48 and 72 hours and crocidolite in 72 hours. 2. Silica, asbestos and man-made mineral fibers (CF, GF) showed the characteristics of apoptosis, DNA ladder, which was induced by incubating A549 cell with each particles for 24 hours in vitro 3. Apoptotic alveolar macrophage was observed the findings of zeiosis (membrane blebbing), condensation of nuclear chromosome and many vacuoles in cytoplasm, electomicroscopically.

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Occupational Cancers with Chemical Exposure and their Prevention in Korea: A Literature Review
Kyung-Taek Rim
Asian Pacific Journal of Cancer Prevention.2013; 14(6): 3379. CrossRef
The Effects of Air-borne Particulate Matters on the Alveolar Macrophages for the TNF-α and IL-1β Secretion
Tian Zhu Li, Soo-Jin Lee, Se-Jong Park, Byung-Joon Chang, Jong-Hwan Lee, Kil-Soo Kim, Myoung-Heon Lee, Nong-Hoon Choe
Tuberculosis and Respiratory Diseases.2006; 60(5): 554. CrossRef

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Relationship Between Mineral Fiber-Induced Pulmonary Tissue Reaction and MIP-2: Kyoung Ah Kim, Young Lim, Dong Won Lee, Heung Nam Kim, Hwang Sin Chang, Ji Hong Kim, Hwa Suck Suh, Chung Yill Park, Im Goung Yun; Korean Journal of Occupational and Environmental Medicine 1997;9(4):650-658. Published online December 31, 1997; DOI: https://doi.org/10.35371/kjoem.1997.9.4.650

Abstract PDF: The pulmonary recruitment and activation of inflammatory cells, in particular, neutrophils is thought to contribute to lung injury resulting from dust exposure. MIP-2 (macrophage inflammatory protein-2) which is a member of C-X-C chemokine plays a key role in neutrophil recruitment to sites of tissue injury. Especially, mineral fiber induced pulmonary response is as a model for the neutrophil recruitment. Therefore, we evaluated the distribution of MIP-2 expression in lung tissue of mineral fiber exposed rat using immunohistochemical study and the relationship between degree of inflammation of lower respiratory tract and MIP-2 expression. Total cell counts in bronchoalveolar lavage (BAL) fluid in mineral fiber-exposed group were markedly increased compared with each control group even not in ceramic fiber group. Number of neutrophil in BAL fluid in mineral fiber-exposed group were markedly increased compared with each control group until 4th week but except ceramic fiber group. In chrysotile group, number of neutrophil in BAL fluid were markedly increased compared with control group at 8th week. Lung tissue instilled with all kinds of mineral fibers showed remarkable developments of bronchus associated lymphoid tissue (BALT) and small multiple granulomas but not for ceramic fiber group. In chrysotile group, multiple granuloma and inflammatory change were more profuse response compared with other groups. MIP-2 was predominently expresses in epithelial cells of bronchioles and bronchus and was express also found in macrophages with lung section at 1 week after fiber instillation. Small amount of epithelial cell associated MIP-2 was present in chrysotile at 8 week group. But MIP-2 was not seen in epithelial cells and macrophages in the lung tissue instilled with crocidolite, ceramic fiber and glass fiber at 8 weeks. Our finding suggest that MIP-2 is predominantly expressed in bronchial epithelial cells of lung from mineral fiber-exposed rat and correlated with inflammatory cell, especially neutrophil, recruitment and tissue reaction. And we documented that MIP-2 expression and neutrophil recruitment in man-made vitreous fiber-exposed rat, especially glass fiber, less than chrysotile.

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The Effect of Hydroxyl Radical Generation on the Cytotoxicity of Alveolar Macrophage and Lipid Peroxidation of Erythrocyte Membrane by Mineral Fibers: Kyoung Ah Kim, Young Lim, Ji Hong Kim, Hwang Sin Chang, Chung Yill Park, Im Goung Yun, Min Hwa Oh; Korean Journal of Occupational and Environmental Medicine 1997;9(3):401-410. Published online October 31, 1997; DOI: https://doi.org/10.35371/kjoem.1997.9.3.401

Abstract PDF: No abstract available.

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The Measurement of IL-1, 8, TNF for the Diagnosis of Pneumoconiosis: Young Lim, Kyoung Ah Kim, Im Goung Yun; Korean Journal of Occupational and Environmental Medicine 1997;9(1):17-25. Published online February 28, 1997; DOI: https://doi.org/10.35371/kjoem.1997.9.1.17

Abstract PDF

Many acute and chronic lung diseased including pneumoconiosis are characterized by the presence of increased numbers of activated macrophages. These macrophages generate several inflammatory cell chemoattractants, by which neutrophil migrate from vascular compartment to the alveolar space. Recruited neutrophils secrete toxic oxygen radicals or proteolytic enzymes and induce inflammatory response. Continuing inflammatory response results in alteration of the pulmonary structure and irreversible fibrosis. Recently, a polypeptide with specific neutrophil chemotactic activity, interleukin-8 (IL-8), has been cloned and isolated from a number of cells such as: monocytes, macrophages and fibroblasts. IL-1 and/or TNF-alpha preceded for the synthesis of IL-8, and we already observed high level of IL-1 and TNF-alpha in vivo experiments. So we hypothesized that IL-8 might play a central role in the pathogenesis of pneumoconiosis. In order to evaluate the clinical utility of IL-1, IL-8, TNF as a biomarker in the early diagnosis of pneumoconiosis, we investigated the increase of IL-1, 8, TNF in the pneumoconiotic patient and the significant (p<0.05) correlation between IL-8 level and progression of pneumoconiosis.

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The Effects of Air-borne Particulate Matters on the Alveolar Macrophages for the TNF-α and IL-1β Secretion
Tian Zhu Li, Soo-Jin Lee, Se-Jong Park, Byung-Joon Chang, Jong-Hwan Lee, Kil-Soo Kim, Myoung-Heon Lee, Nong-Hoon Choe
Tuberculosis and Respiratory Diseases.2006; 60(5): 554. CrossRef

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Effect of Asbestos on Fibroblast Proliferation of Rat: Kyoung Ah Kim, Dong Won Lee, Young Lim, Im Goung Yun; Korean Journal of Occupational and Environmental Medicine 1996;8(3):392-402. Published online December 31, 1996; DOI: https://doi.org/10.35371/kjoem.1996.8.3.392

Abstract PDF

Asbestosis is a chronic inflammatory disorder of lower respiratory tract in which alveolar wall are progressively thickened by a fibrotic process. Fibrotic process characterized by an expansion of fibroblast and collagenous extracellular matrix secreted from this fibroblast. Alveolar macrophage is believed to be a primary target cell and major participant in the evolution of lung fibrosis after asbestos inhalation. Alveolar macrophage are known to release a variety of substance that induce tissue damage and stimulate inflammatory cells and fibroblast. Macrophage also release a variety of metabolite of arachidonic acid. Of these, PGE(2) is known to suppress fibroblast proliferation. Asbestos may be a very effective stimulus for fibroblasts without triggering the relase of PGE(2). To assess the fibrogenic properties of asbestos according to kind and dosage of asbestos and the ability of PGE(2) to suppress the proliferation of fibroblast, alveolar macrophages cultured with crocidolite, amosite and chrysotile in presence or absence of PGE(2)10(-5)M. At 24 hours after alveolar macrophage cultured with various stimuli, the released fibronectin and TNF-alpha was measured. Viability of alveolar macrophages was observed and growth promoting activity of macrphage supernatant to fibroblasts was quantified. The results were as follows; 1. The viability of alveoair macrophages stimulated with asbestos fiber was markedly decreased compared with control group except chrysotile 10 microgram group. Crocidolite and amosite were more cytotoxic than chrysotile. 2. All of asbestos augmented fibronectin production in concentration dependent fashion. 3. There was a significant positive correlation between TNF-alpha production in supernatant and fiber concentration. 4. Supernatant from alveolar macrophages cultured with asbestos were inducible a significant increase in fibroblast proliferation. 5. Incubation of avieolar macrophages with asbestos in the presence of PGE(2) resulted in significant decrease of TNF-alpha production in supernant. 6. Supernatant from alveolar macrophages cultured with asbestos were inducible a: sig nificnat decrease in fibroblast proliferation when PGE(2) was added. The result of this study strongly suggested that crocidolite and amosite were more cytotoxic and fibrogenic and exogenous PGE(2) suppressed fibroblast proliferation following exposed to asbestos.

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The Effects of Air-borne Particulate Matters on the Alveolar Macrophages for the TNF-α and IL-1β Secretion
Tian Zhu Li, Soo-Jin Lee, Se-Jong Park, Byung-Joon Chang, Jong-Hwan Lee, Kil-Soo Kim, Myoung-Heon Lee, Nong-Hoon Choe
Tuberculosis and Respiratory Diseases.2006; 60(5): 554. CrossRef

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Chronic Hypersensitivity Pneumonitis in a Smelter: Kyoung Ah Kim, Young Lim, Im Goung Yun; Korean Journal of Occupational and Environmental Medicine 1995;7(2):230-234. Published online October 31, 1995; DOI: https://doi.org/10.35371/kjoem.1995.7.2.230

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Occupational lung diseases caused by metal oxide are classified as follows, metal fume fever, chemical pneumonitis, hypersensitivity pneumonitis, and occupational asthma. Even though it's well known the immunologic mechanism is involved in metal fume fever, the exact cause is unkown yet. Some authors have speculated that metal fume fever is a form of hypersensitivity pneumonitis. A smelter exposed to metal oxide fumes was hospitalized with some complaints such as severe cough, dyspnea and fever. Chest radiographs on admission showed ill-defined reticulonodular density especially prominent in the base of the both lower lobe. HRCT of chest was observed diffuse fine granolar and reticular involvement with multiple small honeycomb cystic lesion. Bronchoalveolar lavage showed a lymphocyte predominant alveolitis and TBLB had the finding of noncaseating granuloma infiltrating with giant cells, lymphocytes and macrophages. This is a case of alveolitis caused by metal oxide fume which has the characteristics of hypersensitivity pneumonitis in clinical picture and the finding of BAL and TBLB.

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Association of Exposure to Chemicals with Dyspnea among Employed Workers: Analysis of the 3rdKorean Working Conditions Survey
Moon-Young Park, Sung-Ho Hwang, Kimyong Hong, Se-Eun Oh, Kyoung-Mu Lee
Journal of Korean Society of Occupational and Environmental Hygiene.2016; 26(1): 64. CrossRef

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A follow-up study on diatomaceous earth pneumoconiosis in the diatomite factory: Hyun Sul Lim, Ji Yong Kim, Won Jae Lee, Im Goung Yun; Korean Journal of Occupational and Environmental Medicine 1993;5(2):195-204. Published online September 30, 1993; DOI: https://doi.org/10.35371/kjoem.1993.5.2.195

Abstract PDF: No abstract available.

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Rheumatoid factor in coal workers' pneumoconiosis: Jee Won Kim, Im Goung Yun; Korean Journal of Occupational and Environmental Medicine 1993;5(2):187-194. Published online September 30, 1993; DOI: https://doi.org/10.35371/kjoem.1993.5.2.187

Abstract PDF: No abstract available.

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Epidemiological survey on the environment and health status in asbestos factories: Im Goung Yun, Chung Yill Park, Won Chul Lee, Young Lim, Kyung Ah Kim; Korean Journal of Occupational and Environmental Medicine 1993;5(1):137-151. Published online February 28, 1993; DOI: https://doi.org/10.35371/kjoem.1993.5.1.137

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No abstract available.

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Ovarian cancer in a former asbestos textile factory worker: a case report
Sunwook Park, Jaechan Park, Eunsoo Lee, Huisu Eom, Mu Young Shin, Jungwon Kim, Dongmug Kang, Sanggil Lee
Annals of Occupational and Environmental Medicine.2018;[Epub] CrossRef
A Study on Characteristics of Airborne Asbestos Concentrations Using PCM and TEM in Life Environment Surroundings of Seoul
Jinhyo Lee, Suhyun Lee, Jihui Kim, Seokryul Oh, Jinho Shin, Seokwon Eom, Youngzoo Chae, Jinsook Lee, Jayong Koo
Journal of Korean Society of Environmental Engineers.2013; 35(9): 613. CrossRef
Asbestos-related Diseases among Asbestos Textile Factory Workers and Residents Around the Factory
Dong-Mug Kang, Dong-Chul Gu, Kun-Hyung Kim
Journal of the Korean Medical Association.2009; 52(5): 482. CrossRef
Compensation and Diagnosis of Asbestos Related Disease
Soo-Geun Kim
Korean Journal of Family Medicine.2009; 30(5): 335. CrossRef

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Development of diatomaceous earth pneumoconiosis in the diatomitefactory: Hyun Sul Lim, Won Jae Lee, Im Goung Yun; Korean Journal of Occupational and Environmental Medicine 1992;4(1):61-69. Published online February 29, 1992; DOI: https://doi.org/10.35371/kjoem.1992.4.1.61

Abstract PDF: No abstract available.

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Factor pattern of early diagnostic findings in coalworker' pneumoconiosis: Young Lim, Im Goung Yun, Seung Han Lee; Korean Journal of Occupational and Environmental Medicine 1992;4(1):45-51. Published online February 29, 1992; DOI: https://doi.org/10.35371/kjoem.1992.4.1.45

Abstract PDF: No abstract available.

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A case of pulmonary fibrosis after exposure to ammonia gas: Hyeon Woo Yim, Young Lim, Im Goung Yun, Min Hwa Oh; Korean Journal of Occupational and Environmental Medicine 1991;3(2):227-234. Published online November 30, 1991; DOI: https://doi.org/10.35371/kjoem.1991.3.2.227

Abstract PDF: No abstract available.

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A case of Raynaud's syndrome in worker exposed to hand-arm vibration: Kyoung Ah Kim, Hyeon Woo Yim, Young Lim, Im Goung Yun; Korean Journal of Occupational and Environmental Medicine 1991;3(1):119-123. Published online February 28, 1991; DOI: https://doi.org/10.35371/kjoem.1991.3.1.119

Abstract PDF: No abstract available.

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Screening of Respiratory Impairments in Anthracosis: Chee Kyung Chung, Im Goung Yun; Korean Journal of Occupational and Environmental Medicine 1990;2(1):93-104. Published online February 28, 1990; DOI: https://doi.org/10.35371/kjoem.1990.2.1.93

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No abstract available.

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Annual Changes of Lung Function in Retired Workers Exposed to Inorganic Dusts
Joung Oh Lee, Byung-Soon Choi, Jong-Seong Lee, Jee Yeon Jeong, Hong Ki Lee
Tuberculosis and Respiratory Diseases.2011; 71(5): 341. CrossRef

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Lead Poisoning by Administration of Herb Medicine: Yong Zun Kim, Kyung Ah Kim, Young Lim, Im Goung Yun; Korean Journal of Occupational and Environmental Medicine 1990;2(1):64-70. Published online February 28, 1990; DOI: https://doi.org/10.35371/kjoem.1990.2.1.64

Abstract PDF: No abstract available.

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Relations Between Alcohol Intake and Certain Liver Function Teats in Healthy Adult Male White Color Workers: Chung Yill Park, Won Chul Lee, Chee Kyung, Im Goung Yun, Seung Han Lee; Korean Journal of Occupational and Environmental Medicine 1989;1(1):127-139. Published online February 28, 1989; DOI: https://doi.org/10.35371/kjoem.1989.1.1.127

Abstract PDF: We analysed the multivariate relationship between alcohol intake and serum gamma glutamyl-transpeptidase(S-GGTP), aspartate aminotransferase(S-ASAT), alinine aminotransferase(S-ALAT) and age. A group of 1,351 healthy male white color workers aged 18 to 59 years were systematically examined. Weekly alcohol intake and duration was obtained by an interview. The results are as follows: 1. Weekly alcohol intake of 18-29 age groups was significantly less than that of other age group each. And no differences between other age groups can be seen. 2. Statistical analysis of variance showed that the differences found between the various drinking groups was significant for the mean S-GGTP, S-ASAT(P<0.01). 3. From the weekly alcohol intake 180-269 g group, significantly higher values of S-GGTP was found, whereas S-ASAT and S-ALAT were not. 4. Statistical chi-square trends test showed that the difference found between the various drinking groups was significant for the percentage of abnormal S-GGTP, S-ASAT and S-ALAT (P<0.01). 5. Fifty-five percent of the raised values of S-GGTP found between had no identifiable clinical or biochemical abnormality apart from a raised S-GGTP. Fifty-six percent of these otherwise normal subjects came from 31.7% who were moderate or heavy drinkers. It is suggested that the determination of S-GGTP might have value as a screening test for alcoholism. 6. Significant correlations have been found between weekly alcohol intake and corresponding S-GGTP, S-ASAT and S-ALAT values, 0.33, 0.20 and 0.26. respectively. 7. Multiple regression analyses confirmed the superiority of S-GGTP over S-ALAT and S-ALAT as a laboratory marker of alcohol intake and showed the advantage of using S-GGTP and S-ALAT together.

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